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OBJECTIVE:To extract the feature of Achyranthes bidentata and Cyathula officinalis,and to establish image recog-nition method. METHODS:The microscopic image stitching of A. bidentata and C. officinalis was implemented by MATLAB. The color,invariant moment,stripes and the features of vascular bundle in cross section were extracted. The data was organized into da-ta matrix,and then data matrix was standardized by Zscore function;principal components were analyzed through Princomp func-tion. BP nerve network recognition mode was adopted. RESULTS:The microstructures in the micro images of the samples were kept integrated. The measured data of 27 characteristics were acquired in each group of sample. Through principal component analy-sis,the parameters of 11 main components were selected to establish BP never network. The average recognition rate of BP nerve network was 100% between 2 medicinal material relatives (n=50). CONCLUSIONS:The method can be used for micro-image auto Stitching of Chinese medicinal materials and image recognition of A. bidentata and C. officinalis.
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OBJECTIVE:To discuss the improvement in Soxhlet extraction with ore and shell hard Chinese meteria madica taking Plastrum Testudinis as example.METHODS:On the basis of normal Soxhlet extracter,larger Soxhlet extracter on Plastrum Testudinis was experimented using saturated salt-water-bath,quadruplicity cover circle and timer.Large Soxhlet extracter was compared with normal Soxhlet extracter and stirring-reflux method by HPLC analysis,yield and clearance rate of DPPH.RESULTS:There were no significant difference between normal and larger Soxhlet extracters,but the stirring-reflux method was inferior to these two methods.CONCLUSION:The improved larger Soxhlet extraction is a thorough,high-efficient,convenient,economical and healthy extracting method for ore and shell hard Chinese materia medica.
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The Organic Chemistry textbook for traditional Chinese medicine should be connected with this speciality and possese its own characteristic. First,the content in textbook should be chosen according to this specility,so that some outlying teaching content irrelevant to the speciality can be deleted but some correlated content be added to the textbook. Secondly,a lot of examples from traditional Chinese medicine should be inserted in the textbook,especially at "Classification and nomenclature","Important compounds"and"Exercise".
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OBJECTIVE:To establish the method to identify the polysaccharide element in Holothuria atra Jeager by spectroscopy.METHODS:The purity of polysaccharide in Holothuria atra Jeager was determined by electrophoretic method;the infrared absorption spectrum,ultraviolet absorption spectrum,carbon spectra and hydrogen spectra of NMR were identified in structure by infrared spectrophotometry,ultraviolet spectrophotometry and NMR spectrometry respectively.RESULTS:The gel band after electrophoresis took blue color and the results of spectrum determination accountable for each other.CONCLUSION:Uniformly constituent of polysaccharose was found in the Holothuria atra Jeager,which was an acidic polysaccharose with vitriolic esterfunction,without glucopeptide;?-configuration,?-glucopyranose was found and the intramolecular hydrogen bond was formed by hydroxide radical.
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Objective To observe the free radical scavenging action of volatile oil from Piper longum L.growing in Hainan Province and to explore the relationship of the action with molecular structure.Methods 1,1-Diphenyl-2-picrylhydrazyl radical(DPPH) method was used to determine the scavenging action.The components of volatile oil were analyzed by GC-MS,and their molecular structures were identified by databank.Results(1)The volatile oil from Piper longum L.had scavenging actions on free radicals in a concentration-dependent manner.(2)Forty one compounds were found in volatile oil from Piper longum L.growing in Hainan Province,in which the amount of carbon-carbon double bond(C=C) was in predomination.Conclusion Carbon-carbon double bond(C=C)is the essential functional group for free radical scavenging action of volatile oil from Piper longum L.